|Description & NIST Traceability||0.020% v/v solution of toluene in methanol, permanently sealed by heat fusion into a far UV quartz cell. Supplied with a methanol blank. Traceable to NIST SRMs and supplied with UKAS ISO/IEC 17025 accredited certificate of calibration.|
|Primary Usage||Qualification of UV spectrophotometers for bandwidth (resolution) in derivative spectroscopy|
|Useable Range||230 to 290 nm|
|Physical Configuration||Far UV quartz cells that have been permanently sealed by heat fusion|
This reference is described in the European Pharmacopoeia as a resolution test for use in derivative spectroscopy.
In derivative spectroscopy, absorption spectra are transformed into first- second- or higher-order derivatives. This technique facilitates the resolution of two or more peaks that overlap and become merged into a composite curve, and are represented by shoulders on that curve rather than as individual peaks. By calculating the derivative, the underlying individual spectra can be more readily identified.
The reference consists of 0.020% v/v solution of toluene in methanol, permanently sealed by heat fusion into a far UV quartz cell and supplied with a methanol blank.
Suggestions for Use:
The spectrophotometer to be tested must have the ability to produce second-order derivative spectra. The procedure will be described in the instrument’s operating manual.
Scan the toluene in methanol reference cell in second-derivative mode using the methanol cell as a blank. The derivative spectrum should be similar to that shown, with a small negative extremum (-->) between two larger negative extrema at 261 nm and 268 nm.
To comply with the requirements of the European Pharmacopoeia, the ratio A/B should be not less than 0.2.
If the test fails, a service technician may be required to investigate and correct the problem. In any case, repeating the test at intervals will allow the creation of an audit trail that will assist with certification and the rectification of any instrument problems.
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