|Description & NIST Traceability||Quartz cell filled with toluene in hexane and permanently sealed by heat fusion, NIST traceable complete with UKAS ISO/IEC 17025 accredited certificate of calibration|
|Primary Usage||Determination of bandwidth and resolution in the UV region|
|Useable Range||267 to 270 nm|
|Physical Configuration||Far UV quartz cells that have been permanently sealed by heat fusion|
The Spectral Bandwidth (SBW) of a spectrophotometer is the basis of establishing its ability to resolve spectral features separated by small differences in wavelength. If the spectrum of a sample to be measured is suspected of having an interfering peak within the stated spectral bandwidth of the instrument then it should be established whether or not the spectrophotometer is able to resolve the two peaks separately or “mixes” the two absorption peaks into a single peak, leading to erroneous results.
Toluene in hexane is used as a reference for the calculation of SBW. The ratio of the absorbance values of the solution at the peak at 268.7 nm and the trough at 266.8 nm relates directly to the SBW of the instrument being assessed. Regular use of this technique will ensure that the resolution of your instrument is within the required range for your work.
The toluene in hexane reference consists of two far UV quartz spectrophotometer cells with the solution permanently sealed in the cells by heat fusion. One cell contains 0.02% toluene in hexane, the other is a hexane only blank. The Toluene in hexane cell is traceable to SRM 935a which is a transmittance/ absorbance reference material.
Table of Approximate Ratios:
Suggestions for Use:
Scan the toluene in hexane cell against the hexane blank cell from 265 nm to 270 nm at a bandwidth setting appropriate to your analysis. Calculate the ratio of the absorbance values at the peak at 268.7 nm and the trough at 266.8 nm and compare the result with the values in the certificate to determine the spectral bandwidth of your instrument. If the spectral bandwidth is greater than the specification given in your instrument manual, a service technician may be required to investigate and correct the problem.
Periodically scan with the same instrument configuration and compare the results. Over time you will have a data trail for your instrument that will make the detection and correction of any problems relating to spectral bandwidth much more reliable.
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